| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARTMAN, Lauren | SR | Crystal City High School | 2:40.18 | Verified |
| 2 | SANSEGRAW, Celeste | SR | Potosi High School | 2:43.10 | Verified |
| 3 | DRYER, Grace | FR | Pacific High School | 2:45.88 | Verified |
| 4 | MOSS, Carlee | JR | Potosi High School | 2:46.96 | Verified |
| 5 | IJAMES, Izzabelle | JR | Cuba High School | 2:47.79 | Verified |
| 6 | HEETER, Allie | JR | Potosi High School | 2:53.32 | Verified |
| 7 | WENGER, Madeline | SO | Rosati-Kain High School | 2:54.09 | Verified |
| 8 | LAURINE, Elizabeth | JR | Rosati-Kain High School | 2:55.66 | Verified |
| 9 | GEBHART, Mia | JR | Bourbon High School | 2:58.61 | Verified |
| 10 | RICKEY, LylleyAnn | SO | Grandview-R2 (Hillsboro) High Sc | 3:01.00 | Verified |
| 11 | JONES, Ruby | FR | Pacific High School | 3:05.94 | Verified |
| 12 | HARDCASTLE, Patricia | FR | Cuba High School | NT | Unverified: |