| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | RICKEY, LylleyAnn | SO | Grandview-R2 (Hillsboro) High Sc | 1:02.00 | Verified |
| 4 | COLEMAN, Carley | FR | Potosi High School | 58.25 | Verified |
| 5 | SHARP, Theadora | JR | Cuba High School | 1:00.99 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | GIBSON, Kya | SR | Potosi High School | 54.85 | Verified |
| 3 | PATTERSON, Savannah | SR | Grandview-R2 (Hillsboro) High Sc | 53.85 | Verified |
| 4 | LAY, Alexis | JR | Pacific High School | 50.82 | Verified |
| 5 | EDMONDS, Arionna | FR | Pacific High School | 52.69 | Verified |
| 6 | NAHLIK, Faith | FR | Grandview-R2 (Hillsboro) High Sc | 54.85 | Unverified: |
| 7 | BARTLE, Raylee | SO | Bourbon High School | 57.50 | Verified |