| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BODNAR, David | FR | Grandview-R2 (Hillsboro) High Sc | 1:15.30 | Unverified: |
| 3 | LIRETTE, Elijah | FR | Pacific High School | 1:05.71 | Verified |
| 4 | SHEETS, Brayden | FR | Pacific High School | 1:03.85 | Verified |
| 5 | MORALES, Tristan | SR | Crystal City High School | 1:04.99 | Verified |
| 6 | JONES, Max | FR | Cuba High School | 1:06.24 | Verified |
| 7 | CARRICO, Noah | SR | Pacific High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HINOJOSA, Houston | SR | Cuba High School | 57.07 | Verified |
| 2 | GIBSON, Tanner | SR | Potosi High School | 56.94 | Unverified: |
| 3 | REEVES, Zackary | JR | Bourbon High School | 56.72 | Verified |
| 4 | HAYNES, Royce | SR | Grandview-R2 (Hillsboro) High Sc | 54.37 | Verified |
| 5 | KEIM, Wyatt | FR | Grandview-R2 (Hillsboro) High Sc | 55.38 | Verified |
| 6 | MOSIER, Gage | SR | Potosi High School | 56.93 | Verified |
| 7 | HALE, Garrett | JR | Potosi High School | 56.95 | Unverified: |
| 8 | DEROUSSE, Landynn | FR | Crystal City High School | 57.18 | Verified |