| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WENGER, Madeline | SO | Rosati-Kain High School | 1:18.50 | Unverified: estimate |
| 4 | IJAMES, Izzabelle | JR | Cuba High School | 1:12.15 | Verified |
| 5 | WIENKE, Elyse | SO | Grandview-R2 (Hillsboro) High Sc | 1:13.10 | Verified |
| 6 | RIEGLER, Adeline | FR | Pacific High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLONTS, Cierra | JR | Bourbon High School | 1:09.78 | Verified |
| 2 | REED, Bailey | SO | Pacific High School | 1:08.03 | Verified |
| 3 | DANIEL, Aleyna | SR | Pacific High School | 1:03.10 | Verified |
| 4 | WAKELAND, Catherine | SO | Grandview-R2 (Hillsboro) High Sc | 1:01.36 | Verified |
| 5 | WAKELAND, Anna Belle | SR | Grandview-R2 (Hillsboro) High Sc | 1:02.18 | Verified |
| 6 | ENKE, Marisa | SO | Bourbon High School | 1:06.71 | Verified |
| 7 | ELSTON, Brooke | SR | Rosati-Kain High School | 1:09.58 | Verified |
| 8 | BLACK, Rylee | JR | Cuba High School | 1:09.85 | Verified |