| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Grandview-R2 (Hillsboro) High Sc | A | Maxwell | Ottolini | Bodnar | Fox | 1:00.01 | Unverified: |
| 3 | Cuba High School | A | Dake | Halbert | Volner | Hinojosa | 48.29 | Verified |
| 4 | Crystal City High School | A | Compton | DeRousse | Mayes | Bolton | 46.28 | Verified |
| 5 | Potosi High School | A | Neubrand | Sprous | Mosier | Walton | 47.45 | Verified |
| 6 | Pacific High School | A | Magsigay | Thomas | Avilez | Krug | 48.89 | Verified |