| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Crystal City High School | A | Edwards | Dugal | Partney | Thurman | NT | Unverified: |
| 3 | Cuba High School | A | Viramontes | Lunsford | Martellaro | Dunham | 1:00.59 | Verified |
| 4 | Grandview-R2 (Hillsboro) High Sc | A | Boker | McClune | Nahlik | Morris | 54.54 | Verified |
| 5 | Potosi High School | A | McCloud | Coleman | Randall | Randall | DNS | Verified |
| 6 | Pacific High School | A | Bonner | Edmonds | Murphy | Huerta | 1:01.61 | Verified |
| 7 | Rosati-Kain High School | A | Cole | Elston | Hemphill | Smith | NT | Unverified: |