| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Dominic High School | A | Koenig | Powell | Wood | Penning | NT | Unverified: |
| 4 | Ft. Zumwalt West High School | A | 3:40.00 | Unverified: | ||||
| 5 | Christian High School | A | Schroer | States | Thompson | Hardwicke | NT | Unverified: |
| 6 | Ft. Zumwalt North High School | A | Figg | Page | Friend | Miller | NT | Unverified: |