| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAUPIN, Sophia | JR | Ft. Zumwalt West High School | 2:32.73 | Verified |
| 2 | MCGRATH, Katherine | SO | Ft. Zumwalt West High School | 2:35.78 | Verified |
| 3 | OSTERHOLT, Megan | JR | St. Dominic High School | 2:42.11 | Verified |
| 4 | MCDANIEL, Karsyn | SO | Ft. Zumwalt West High School | 2:45.98 | Verified |
| 5 | BUSBY, Lillian | JR | St. Dominic High School | 2:46.45 | Verified |
| 6 | JONES, Peyton | SO | Ft. Zumwalt North High School | NT | Unverified: |
| 7 | WOYTUS, Abigail | SO | St. Dominic High School | NT | Unverified: |