| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FERNANDEZ, Ava | SO | St. Dominic High School | NT | Unverified: |
| 2 | JONES, Mena | SO | St. Dominic High School | NT | Unverified: |
| 3 | RAWLS, Sarah | SO | Ft. Zumwalt West High School | 58.86 | Verified |
| 4 | WASHINGTON, Madison | SO | Ft. Zumwalt West High School | 52.49 | Verified |
| 5 | MAIER, Savannah | JR | Ft. Zumwalt North High School | 55.77 | Verified |
| 6 | PRITT, Ava | SO | St. Dominic High School | 1:01.25 | Verified |
| 7 | CORWIN, Ella | JR | Christian High School | NT | Unverified: |
| 8 | JENNINGS, Makenna | SO | Ft. Zumwalt West High School | NT | Unverified: |