| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WOOD, Henry | SO | St. Dominic High School | NT | Unverified: |
| 3 | POTTS, Will | 9 | Christian High School | NT | Unverified: |
| 5 | FRIEND, Antoine | JR | Ft. Zumwalt North High School | 1:05.09 | Unverified: |
| 6 | POWELL, Cody | SR | St. Dominic High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JACOBS, Katsuo | SO | Christian High School | 58.21 | Verified |
| 2 | PAGE, Noah | SR | Ft. Zumwalt North High School | 54.77 | Verified |
| 3 | SCHROER, Judah | SO | Christian High School | 54.48 | Verified |
| 4 | JONES, Alex | JR | Ft. Zumwalt West High School | 52.47 | Verified |
| 5 | KOENIG, Samuel | JR | St. Dominic High School | 54.39 | Verified |
| 6 | PENNING, Jared | JR | St. Dominic High School | 54.67 | Verified |
| 7 | GREEN, Jy'Ren | SO | Ft. Zumwalt West High School | 55.00 | Unverified: |
| 8 | FIGG, Ayden | JR | Ft. Zumwalt North High School | 1:00.08 | Verified |