| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | ZEIGLER, Heidi | 9 | St. Dominic High School | NT | Unverified: |
| 4 | JONES, Peyton | SO | Ft. Zumwalt North High School | NT | Unverified: |
| 5 | PAINTER, Olivia | SO | Christian High School | NT | Unverified: |
| 6 | KIMBLE, Taylah | JR | Ft. Zumwalt North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STEIMEL, Elle | SO | Christian High School | 1:10.35 | Verified |
| 2 | BURLE, Grace | SR | St. Dominic High School | 1:04.99 | Verified |
| 3 | ANDERSON, Oakley | SO | Ft. Zumwalt West High School | 1:04.58 | Verified |
| 4 | EBERT, Jailyn | JR | Ft. Zumwalt North High School | 1:03.46 | Verified |
| 5 | RIDER, Lyla | SR | Ft. Zumwalt West High School | 1:04.12 | Verified |
| 6 | RYAN, Abigail | SR | St. Dominic High School | 1:04.78 | Verified |
| 7 | MCDANIEL, Karsyn | SO | Ft. Zumwalt West High School | 1:09.47 | Verified |
| 8 | PILS, Abby | SO | Christian High School | NT | Unverified: |