| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Dominic High School | A | Overton | Kell | Pulliam | Koenig | NT | Unverified: |
| 4 | Ft. Zumwalt North High School | A | Futrell | Henderson | Jones | Miller | 43.85 | Unverified: |
| 5 | Ft. Zumwalt West High School | A | 46.00 | Unverified: |