| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MASON, Taylor | SR | Ft. Zumwalt North High School | 4:33.05 | Verified |
| 2 | HARDWICKE, Ronan | SR | Christian High School | 4:35.63 | Verified |
| 3 | ST. JEAN, Dominic | JR | Ft. Zumwalt West High School | 4:53.00 | Unverified: |
| 4 | BOUDINOT, Colton | SO | Ft. Zumwalt West High School | 4:57.73 | Verified |
| 5 | O'BRIEN, Jack | JR | Ft. Zumwalt West High School | 4:59.00 | Unverified: |
| 6 | POTOCKI, Adam | SO | Ft. Zumwalt West High School | 5:00.00 | Unverified: |
| 7 | MEYER, Alan | SR | Ft. Zumwalt North High School | 5:06.64 | Verified |
| 8 | WILLENBRINK, Tyler | SR | St. Dominic High School | 5:23.38 | Verified |
| 9 | JACOBS, Katsuo | SO | Christian High School | 5:38.96 | Verified |
| 10 | PIERCE, Kevin | SO | St. Dominic High School | 5:58.09 | Verified |
| 11 | FINK, James | SO | St. Dominic High School | 6:19.44 | Verified |
| 12 | STEINBRUEGGE, John | 9 | St. Dominic High School | NT | Unverified: |