| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Dominic High School | A | Wulkopf | Powell | Phillips | Livers | NT | Unverified: |
| 4 | Ft. Zumwalt North High School | A | Futrell | Pruitt | Henderson | Miller | 1:31.62 | Unverified: |
| 5 | Ft. Zumwalt West High School | A | 1:34.00 | Unverified: |