| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STEEPLES, Brooke | FR | Parkway Central High School | NT | Unverified: |
| 2 | HYMES, Gwen | JR | Cor Jesu Academy | NT | Unverified: |
| 3 | SMITH, Agnes | SO | Kirkwood High School | 24.34 | Unverified: |
| 4 | BUSKER, Arden | JR | Ladue Horton Watkins High School | 23.02 | Verified |
| 5 | TAYLOR, DaMya | SR | Parkway Central High School | 23.56 | Verified |
| 6 | HORTON, Emily | SO | Northwest (Cedar Hill) High Scho | 25.00 | Unverified: |
| 7 | FRAZIER, Alexandria | SO | Parkway South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HERNDON, Divine | SO | Riverview Gardens High School | 22.43 | Verified |
| 2 | SANDERS, Hallie | SO | Lindbergh High School | 22.00 | Unverified: |
| 3 | NORMAN, Kamaya | SO | Webster Groves High School | 21.52 | Verified |
| 4 | BLUE, Olivia | FR | Ladue Horton Watkins High School | 21.14 | Verified |
| 5 | CRINNION, Cameron | FR | Seckman High School | 21.27 | Verified |
| 6 | NOTTINGHAM, Ella | FR | Eureka High School | 21.80 | Unverified: |
| 7 | NORTHCROSS, Demecha | SO | Parkway West High School | 22.23 | Verified |
| 8 | MONTROY, Sierra | FR | Lindbergh High School | 23.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TUBBESING, Elizabeth | SO | Kirkwood High School | 20.78 | Verified |
| 2 | VANDERHOOF, Amara | FR | Eureka High School | 20.30 | Unverified: |
| 3 | SMITH, Breanna | JR | Lindbergh High School | 20.00 | Unverified: |
| 4 | ALDENDERFER, JESSICA | JR | Marquette High School | 19.50 | Unverified: |
| 5 | FORMBY, Sabrina | FR | Seckman High School | 19.93 | Verified |
| 6 | RUSSELL, Sophia | SR | Marquette High School | 20.20 | Unverified: |
| 7 | DOHERTY, GRACE | SO | Lafayette (Wildwood) High School | 20.70 | Unverified: |
| 8 | MU, Sarah | FR | Lafayette (Wildwood) High School | 21.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAI, Huitong | FR | Marquette High School | 19.40 | Unverified: |
| 2 | HAAS, Lexi | SO | Northwest (Cedar Hill) High Scho | 19.20 | Unverified: |
| 3 | HENROID, Lillian | JR | Parkway Central High School | 18.78 | Verified |
| 4 | REECE, Na'Kyia | JR | Hazelwood Central High School | 17.77 | Verified |
| 5 | BOLFING, Gabi | SR | Eureka High School | 18.60 | Unverified: |
| 6 | KOMMU, Lasya | FR | Lafayette (Wildwood) High School | 19.00 | Unverified: |
| 7 | MCFOWLAND, Pamela | SO | Riverview Gardens High School | 19.35 | Verified |
| 8 | WELLS, Callie | JR | Northwest (Cedar Hill) High Scho | 19.50 | Unverified: |