| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vashon High School | A | Cain III | Blockette | McField | Taylor | 3:54.57 | Verified |
| 2 | Marquette High School | A | ANDERSON | CODY-JACKSON | ELLIS | WILLIAMS | DNS | Verified |
| 3 | Liberty (Wentzville) High School | A | Wilkes | Liddell | Eldredge | Jones | 3:38.64 | Unverified: |
| 4 | Hazelwood East High School | A | Johnson | Shakoor | Sledge | Williams | 3:35.05 | Verified |
| 5 | Maplewood-Richmond Hts. High Sch | A | Boone | Gavin | Carter | Clark | 3:38.32 | Unverified: |
| 6 | Parkway West High School | A | Sze | Beach | Beveridge | Moore | 3:39.00 | Unverified: |
| 7 | Northwest (Cedar Hill) High Scho | A | Porter | Riley | Poskin | Campbell | 3:53.44 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | A | Webb | Welch | Robinson | Snyder | 3:34.90 | Unverified: |
| 2 | Poplar Bluff High School | A | Dodd | McBride | Burton | Nunn | 3:33.80 | Unverified: Dexter Openvite |
| 3 | Hazelwood Central High School | A | Johnson | Logan | Donald | Anthony | 3:30.26 | Unverified: |
| 4 | Riverview Gardens High School | A | Jones | Thompson | Howard | Anderson | 3:30.00 | Unverified: |
| 5 | Lafayette (Wildwood) High School | A | STITH | Ems | King | Marshall | 3:30.00 | Unverified: |
| 6 | Ladue Horton Watkins High School | A | Allen | Hawthorne | Levine | Patterson | 3:30.79 | Verified |
| 7 | Ft. Zumwalt North High School | A | Miller | Mason | Ostmann | Page | 3:34.60 | Verified |
| 8 | Seckman High School | A | Gossett | Matus | House | Tilley | 3:35.00 | Unverified: |