| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Marquette High School | A | LEOPANDO | PLEIN | Warden | Bergoudian | 3:56.00 | Unverified: |
| 4 | Ft. Zumwalt North High School | A | ARPASI | Pirrung | Friend | Smith | 3:50.81 | Unverified: Todd Bizzell Memorial "Spartan R |
| 5 | Liberty (Wentzville) High School | A | Lawrence | Sinclair | Lively | Minnix | 3:55.64 | Unverified: |
| 6 | Northwest (Cedar Hill) High Scho | A | Washington | Grellner | Judge | Penberthy | 4:04.93 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | A | Araneta | Earl | Walter | Larson | 3:49.00 | Unverified: |
| 2 | Riverview Gardens High School | A | Caph | McClain | Walker | Bayless | 3:47.27 | Verified |
| 3 | Lafayette (Wildwood) High School | A | Smith | Han | BERRA | Retych | 3:42.00 | Unverified: |
| 4 | Hazelwood East High School | A | Anderson | III | Watson | Pichon | 3:35.05 | Verified |
| 5 | Hazelwood Central High School | A | Bright | Anthony | Nance | Thornton | 3:40.44 | Verified |
| 6 | Maplewood-Richmond Hts. High Sch | A | Willis | Schmitt | Royston | Manipula | 3:44.32 | Verified |
| 7 | Seckman High School | A | Schutte | Smith | Nissen | Mraz | 3:48.00 | Unverified: |
| 8 | Ladue Horton Watkins High School | A | Hawthorne | Griffin | Taylor | Pinex | 3:49.79 | Unverified: |