| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cor Jesu Academy | A | Figliola | Bognar | Goldkamp | Breckenridge | 4:39.40 | Verified |
| 2 | Ft. Zumwalt North High School | A | Ebert | Abdelkarim | Maier | Jones | 4:32.40 | Verified |
| 3 | Northwest (Cedar Hill) High Scho | A | Surratt | Orozco | Griffin | Stethem | 4:30.00 | Unverified: |
| 4 | Poplar Bluff High School | A | Paumen | Hampton | Stotts | Kramer | DNS | Verified |
| 5 | Parkway West High School | A | Fotheringham | Watts | Etchason | Grunzinger | 4:30.00 | Unverified: |
| 6 | Marquette High School | A | CLARK | EHIMARE | Schweitzer | QIN | 4:30.00 | Unverified: |
| 7 | Oakville High School | A | JONAS | BROWER | WESCOAT | SQUIRES | 4:33.65 | Verified |
| 8 | Lindbergh High School | A | Kelley | Rea | Cintel | Forbes | 4:40.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty (Wentzville) High School | A | Omara | Kalbac | Helm | Kammermeyer | 4:25.38 | Verified |
| 2 | Lafayette (Wildwood) High School | A | Singh | Pauls | Brown | Dantuono | 4:25.00 | Unverified: |
| 3 | Parkway Central High School | A | Fritz | Green | Benoist | Harris | 4:18.42 | Unverified: McCullough-Douglas Invitational |
| 4 | Ladue Horton Watkins High School | A | Buxner | Griffin | Murray | Rogers | 4:09.42 | Verified |
| 5 | Hazelwood Central High School | A | Stamps | Mckenzie | Catching | Elam | 4:18.02 | Unverified: |
| 6 | Riverview Gardens High School | A | Smith | Smith | Gladden | Green | 4:20.00 | Unverified: |
| 7 | Eureka High School | A | DEAKIN | SCHNURBUSCH | Morse | Gentilini | 4:25.00 | Unverified: |
| 8 | Webster Groves High School | A | Decker | King | Logan | Murdock | 4:28.55 | Verified |