| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | A | BOLT | Bhatia | Bolt | Wrisberg | 5:20.00 | Unverified: |
| 2 | Maplewood-Richmond Hts. High Sch | A | Jones | Horton | Robinson | Mohr | 5:04.48 | Verified |
| 3 | Cor Jesu Academy | A | Probst | Shoger | Mose | Pieper | 4:55.00 | Unverified: Holt Invitational |
| 4 | Lindbergh High School | A | Chand | Zoeller | Kobe | Bani | 4:50.00 | Unverified: |
| 5 | Lafayette (Wildwood) High School | A | Nichols | Kilmer | McGinnis | Long | 4:50.00 | Unverified: |
| 6 | Parkway West High School | A | Josef | Phillips | Sanders | Ashok | 5:00.00 | Unverified: |
| 7 | McCluer North High School | A | Jefferson | Clayton | Eubanks | Davis | DNS | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Perry | McCoy | Williams | Lawless | 4:44.65 | Unverified: |
| 2 | Kirkwood High School | A | Martz | Tubbesing | Mitchell | MacLellan | 4:36.51 | Verified |
| 3 | Ft. Zumwalt North High School | A | Carter | Brockhurst | Martin | Bello | 4:32.40 | Verified |
| 4 | Hazelwood Central High School | A | McCanery | Cannon | White | Scruggs | 4:30.02 | Unverified: |
| 5 | Riverview Gardens High School | A | Davis | Coleman | Robinson | Lewis | 4:31.38 | Verified |
| 6 | Eureka High School | A | BRICKMAN | DAFFRON | Contestabile | Wylie | 4:35.00 | Unverified: |
| 7 | Ladue Horton Watkins High School | A | Blue | Ray | Jackson | Small | 4:40.42 | Unverified: |
| 8 | Parkway Central High School | A | Anderson | Tremont | Mosley | Thedford | 4:45.42 | Unverified: McCullough-Douglas Invitational |