| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BACON, Lauren | SO | Parkway South High School | NT | Unverified: |
| 4 | MOSLEY, Timiya | JR | Vashon High School | NT | Unverified: |
| 5 | MADISON, Jamaria | JR | Hazelwood Central High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ULLOM, CiCi | JR | Parkway South High School | NT | Unverified: |
| 2 | PERKINS, Kimberlan | JR | Riverview Gardens High School | NT | Unverified: |
| 3 | HARVELL, Abigail | SR | Seckman High School | 31.43 | Verified |
| 4 | OMARA, Caitlin | JR | Liberty (Wentzville) High School | 29.95 | Verified |
| 5 | DUNLAP, Cadence | JR | Ft. Zumwalt North High School | 31.14 | Verified |
| 6 | WILLIAMS, Carmillya | SR | McCluer North High School | NT | Unverified: |
| 7 | JACOBY, Corinne | SO | Cor Jesu Academy | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THIELE, Samantha | JR | Eureka High School | 29.50 | Unverified: |
| 2 | EARNEST, Emerie | JR | Maplewood-Richmond Hts. High Sch | 29.40 | Verified |
| 3 | GILLS, Ashten | SR | Parkway West High School | 29.30 | Unverified: |
| 4 | WOODS, Sanaa | FR | Liberty (Wentzville) High School | 29.18 | Verified |
| 5 | BOYD, Madison | JR | Vashon High School | 29.22 | Verified |
| 6 | SADDLER, Kaliyah | SR | Lafayette (Wildwood) High School | 29.40 | Unverified: |
| 7 | HAVILAND, Kathleen | SR | Lindbergh High School | 29.50 | Unverified: |
| 8 | KIMBLE, Taylah | JR | Ft. Zumwalt North High School | 29.84 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIXON, Summer | SO | Kirkwood High School | 29.17 | Verified |
| 2 | JOHNSON, Nykira | JR | Parkway West High School | 29.10 | Unverified: |
| 3 | STOECKEL, Anna | SR | Cor Jesu Academy | 29.03 | Verified |
| 4 | TREVINO, Nylla | FR | Webster Groves High School | 28.73 | Verified |
| 5 | BOWMAN, MORGAN | JR | Marquette High School | 29.00 | Unverified: |
| 6 | MUELLER, Jenna | SR | Oakville High School | 29.08 | Verified |
| 7 | JONAS, MADYSEN | JR | Oakville High School | 29.14 | Verified |
| 8 | FRITZ, Zoe | SR | Parkway Central High School | 29.17 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHAFFREY, Lily | SR | Northwest (Cedar Hill) High Scho | 28.60 | Unverified: |
| 2 | MORIE, Lillian | FR | Seckman High School | 28.20 | Unverified: |
| 3 | SMITH, Aikyla | JR | Riverview Gardens High School | 27.88 | Verified |
| 4 | MITCHELL, Hannah | JR | Eureka High School | 27.70 | Unverified: |
| 5 | HIERS, Hannah | SR | Lindbergh High School | 27.80 | Unverified: |
| 6 | TABB, Chloe | FR | Lafayette (Wildwood) High School | 28.00 | Unverified: |
| 7 | SIMON, Mia | FR | Webster Groves High School | 28.37 | Verified |
| 8 | SHELLEDY, Isabella | SO | Northwest (Cedar Hill) High Scho | 28.70 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REED, Carrington | SO | Hazelwood Central High School | 26.86 | Verified |
| 2 | WILLIAMS, Koryenna | JR | Poplar Bluff High School | 26.46 | Verified |
| 3 | DOWD, Samantha | FR | Poplar Bluff High School | 26.09 | Verified |
| 4 | BUCKLEY, Jordyn | SO | McCluer North High School | 25.42 | Verified |
| 5 | GREEN, Mychael | SR | Parkway Central High School | 26.08 | Verified |
| 6 | BRINKER, Delaney | SO | Ladue Horton Watkins High School | 26.19 | Unverified: |
| 7 | DAVIS, SAMARA | JR | Marquette High School | 26.64 | Unverified: |
| 8 | BEAUMONT, Jessie | SR | Kirkwood High School | 27.65 | Verified |