| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NEWSHAM, Olivia | FR | Kirkwood High School | NT | |
| 2 | BOCQUILLON, Rebecca | SO | Lindbergh High School | NT | Unverified: |
| 3 | HYMES, Gwen | JR | Cor Jesu Academy | NT | Unverified: |
| 4 | MONTROY, Sierra | FR | Lindbergh High School | NT | Unverified: |
| 5 | HARRIS, Kayla | SO | Hazelwood Central High School | NT | Unverified: |
| 6 | FRAZIER, Alexandria | SO | Parkway South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HORTON, Emily | SO | Northwest (Cedar Hill) High Scho | 1:12.20 | Unverified: |
| 2 | RASHMAN, Corinne | FR | Marquette High School | 1:04.80 | Unverified: |
| 3 | CHAND, Maya | JR | Lindbergh High School | 1:03.00 | Unverified: |
| 4 | MU, Sarah | FR | Lafayette (Wildwood) High School | 1:02.00 | Unverified: |
| 5 | HERNDON, Divine | SO | Riverview Gardens High School | 1:02.02 | Verified |
| 6 | TUBBESING, Elizabeth | SO | Kirkwood High School | 1:03.22 | Verified |
| 7 | CHAUSOW, Hannah | FR | Parkway Central High School | 1:06.68 | Verified |
| 8 | JONES, Mika | FR | Vashon High School | 1:15.12 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DOHERTY, GRACE | SO | Lafayette (Wildwood) High School | 1:01.00 | Unverified: |
| 2 | NOTTINGHAM, Ella | FR | Eureka High School | 1:00.00 | Unverified: |
| 3 | MITCHELL, Alexis | JR | Kirkwood High School | 59.70 | Verified |
| 4 | MCFOWLAND, Pamela | SO | Riverview Gardens High School | 58.89 | Verified |
| 5 | CRINNION, Cameron | FR | Seckman High School | 59.17 | Verified |
| 6 | HENROID, Lillian | JR | Parkway Central High School | 59.74 | Verified |
| 7 | TREMONT, Cecilia | FR | Parkway Central High School | 1:00.94 | Verified |
| 8 | HAAS, Lexi | SO | Northwest (Cedar Hill) High Scho | 1:02.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KOMMU, Lasya | FR | Lafayette (Wildwood) High School | 58.00 | Unverified: |
| 2 | BLUE, Olivia | FR | Ladue Horton Watkins High School | 56.93 | Verified |
| 3 | FORMBY, Sabrina | FR | Seckman High School | 55.32 | Verified |
| 4 | NELSON, Lillian | JR | Liberty (Wentzville) High School | 54.33 | Verified |
| 5 | REECE, Na'Kyia | JR | Hazelwood Central High School | 55.08 | Verified |
| 6 | VANDERHOOF, Amara | FR | Eureka High School | 56.00 | Unverified: |
| 7 | BOLFING, Gabi | SR | Eureka High School | 57.00 | Unverified: |
| 8 | NABORS, Dezirae' | JR | McCluer North High School | 58.69 | Verified |