| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BOGNAR, Ava | FR | Cor Jesu Academy | NT | Unverified: |
| 4 | BOYD, Madison | JR | Vashon High School | NT | Unverified: |
| 5 | FLEMING, Raven | JR | Kirkwood High School | NT | Unverified: |
| 6 | MOORE, Dyonna | JR | Vashon High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HAMPTON, Shaylin | JR | Poplar Bluff High School | 1:09.39 | Verified |
| 2 | FOTHERINGHAM, Audrey | SO | Parkway West High School | 1:09.10 | Verified |
| 3 | KELLEY, Jeannette | SO | Lindbergh High School | 1:09.00 | Unverified: |
| 4 | SQUIRES, AYDAN | JR | Oakville High School | 1:08.49 | Verified |
| 5 | BOWMAN, MORGAN | JR | Marquette High School | 1:09.00 | Unverified: |
| 6 | BROWN, Taylor | FR | McCluer North High School | 1:09.00 | Unverified: |
| 7 | HARRIS, Armani | FR | Parkway Central High School | 1:09.11 | Unverified: |
| 8 | DRAKE, Makinsey | SR | Parkway West High School | 1:10.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MORSE, Kathryn | SR | Eureka High School | 1:08.34 | Unverified: |
| 2 | ZIMMERMANN, Hailey | JR | Seckman High School | 1:07.94 | Verified |
| 3 | SINGH, Shrutika | SR | Lafayette (Wildwood) High School | 1:07.00 | Unverified: |
| 4 | HIERS, Hannah | SR | Lindbergh High School | 1:07.00 | Unverified: |
| 5 | GREEN, Aniyah | SO | Riverview Gardens High School | 1:07.00 | Unverified: |
| 6 | BRECKENRIDGE, Dylan | FR | Cor Jesu Academy | 1:07.70 | Verified |
| 7 | WESCOAT, ELIZABETH | SO | Oakville High School | 1:08.03 | Verified |
| 8 | EBERT, Jailyn | JR | Ft. Zumwalt North High School | 1:08.34 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ULLOM, CiCi | JR | Parkway South High School | 1:06.29 | Verified |
| 2 | FRITZ, Zoe | SR | Parkway Central High School | 1:06.11 | Verified |
| 3 | JONES, Peyton | SO | Ft. Zumwalt North High School | 1:06.02 | Verified |
| 4 | CATCHING, Aliya | JR | Hazelwood Central High School | 1:04.12 | Unverified: |
| 5 | WILKES, Mariah | JR | Kirkwood High School | 1:04.96 | Verified |
| 6 | TREVINO, Nylla | FR | Webster Groves High School | 1:06.07 | Verified |
| 7 | LOGAN, Teuana | JR | Webster Groves High School | 1:06.18 | Verified |
| 8 | CROOKS, Lydia | SR | Eureka High School | 1:06.55 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SMITH, Alondria | SR | Riverview Gardens High School | 1:03.16 | Verified |
| 2 | SURRATT, Cami | JR | Northwest (Cedar Hill) High Scho | 1:02.50 | Unverified: |
| 3 | BROWN, Avery | SO | Lafayette (Wildwood) High School | 1:00.00 | Unverified: |
| 4 | BUCKLEY, Jordyn | SO | McCluer North High School | 59.80 | Unverified: |
| 5 | GRIFFIN, Karina | SO | Ladue Horton Watkins High School | 59.94 | Unverified: |
| 6 | WARD, Angela | SR | Seckman High School | 1:01.39 | Verified |
| 7 | PAUMEN, Caitlin | SR | Poplar Bluff High School | 1:02.84 | Verified |
| 8 | STAMPS, Ryann | FR | Hazelwood Central High School | 1:04.10 | Verified |