| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Hazelwood East High School | A | Burks | Shakoor | Sledge | Williams | 45.60 | Verified |
| 4 | Seckman High School | A | Tilley | Horton | Gossett | Foster | 45.00 | Unverified: |
| 5 | Marquette High School | A | Jones | BONNETT | JACKSON | ELLIS | DQ | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vashon High School | A | Morris | Phillips | Blockette | Taylor | 44.63 | Unverified: |
| 2 | Ladue Horton Watkins High School | A | Allen | Roberts | Lucas | Levine | 44.20 | Unverified: |
| 3 | Riverview Gardens High School | A | Anderson | Thompson | Howard | Jones | 43.23 | Unverified: |
| 4 | Hazelwood Central High School | A | Atkins | Martin | Williams | Coleman | 43.07 | Unverified: |
| 5 | Poplar Bluff High School | A | McFarland | McBride | Graham | Amos | 43.21 | Unverified: Jackson Invitational |
| 6 | Lafayette (Wildwood) High School | A | JONES | King | STITH | LEWIS | 44.00 | Unverified: |
| 7 | Ft. Zumwalt North High School | A | Futrell | Henderson | Jones | Pruitt | NT | Verified |
| 8 | Kirkwood High School | A | Sloan | Edwards | Smith | Richardson | 45.00 | Unverified: |