| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Marquette High School | A | Cross | DAVIS | HOUCHIN | QIN | NT | Unverified: 53.5 |
| 4 | Riverview Gardens High School | A | Perkins | Green | Jackson | Washington | 57.00 | Unverified: Hazelwood Invitational |
| 5 | Vashon High School | A | Mosley | Riggins | Butler | Boyd | 58.91 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | McCluer North High School | A | Murphy | Goforth | Taylor | Hammond | 55.65 | Unverified: |
| 3 | Northwest (Cedar Hill) High Scho | A | Griffin | G'Sell | Labrash | Shaffrey | 54.50 | Unverified: |
| 4 | Webster Groves High School | A | Harrison | Rucker | Simon | Decker | 54.33 | Verified |
| 5 | Ft. Zumwalt North High School | A | Hamilton | Kimble | Michel | Noel | 54.42 | Verified |
| 6 | Parkway Central High School | A | Benoist | Lee | Owens | Collins-Bey | 55.25 | Verified |
| 7 | Cor Jesu Academy | A | Stoeckel | Jacoby | Lorms | Graham | 55.66 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Seckman High School | A | Morie | Bantle | Harvell | Leitner | 53.00 | Unverified: |
| 2 | Lindbergh High School | A | Thomas | Scott | Lewis | Hiers | 52.00 | Unverified: |
| 3 | Ladue Horton Watkins High School | A | Brinker | Williams | White | Murray | 51.60 | Unverified: |
| 4 | Hazelwood Central High School | A | Madison | Reed | Mckenzie | Wilkins | 49.61 | Verified |
| 5 | Eureka High School | A | DEAKIN | DOSS | SEITZ | MITCHELL | 51.50 | Unverified: |
| 6 | Poplar Bluff High School | A | Williams | Dowd | Kendle | Cassie | 51.90 | Verified |
| 7 | Lafayette (Wildwood) High School | A | White | Witherspoon | Vellines | Tabb | 52.50 | Unverified: |
| 8 | Liberty (Wentzville) High School | A | Helm | OMara | Woods | Kammermeyer | 54.26 | Verified |