| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Northwest (Cedar Hill) High Scho | A | Ambrocio-Valencia | Koehler | Grobelny | Finder | 1:03.00 | Unverified: |
| 4 | Cor Jesu Academy | A | Gremaud | Gremaud | Detweiler | Mathews | 1:00.00 | Unverified: Fort Zumwalt West Jaguar Invitat |
| 5 | Lindbergh High School | A | Lord | Ritter | Mujkanovic | Vogler | 1:01.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | McCluer North High School | A | Eubanks | Moore | Dent | Hart | DNS | Unverified: |
| 2 | Liberty (Wentzville) High School | A | Kemp | Richardson | Long | Johnson | 59.26 | Unverified: |
| 3 | Seckman High School | A | Dundon | Wynn | Mattler | Easter | 58.00 | Unverified: |
| 4 | Kirkwood High School | A | Taylor | Barry | Burgett | Pride | 57.74 | Unverified: |
| 5 | Parkway Central High School | A | Bell | Thedford | Mosley | Madison | 58.00 | Unverified: |
| 6 | Vashon High School | A | Artis-Moore | Conner | Brooks | Hardieway | 58.91 | Verified |
| 7 | Ladue Horton Watkins High School | A | Blue | Busker | Small | Ta | 59.71 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | Alvarez | Gardner | Saddler | Willis | 56.00 | Unverified: |
| 2 | Ft. Zumwalt North High School | A | King | Carter | Williams | Taylor | 54.42 | Verified |
| 3 | Eureka High School | A | Crooks | Bailey | DAFFRON | FRAZIER | 54.00 | Unverified: |
| 4 | Hazelwood Central High School | A | Sheppard | Miles | Wicks | Griffin | NT | Unverified: |
| 5 | Riverview Gardens High School | A | Harris-Grayer | Robinson | Coleman | Lewis | 54.00 | Unverified: Hazelwood Invitational |
| 6 | Webster Groves High School | A | Webb | Williams | Perry | Dill | 54.33 | Verified |
| 7 | Parkway South High School | A | Gavornik | Wright | Cashion | Stroot | 54.62 | Verified |
| 8 | Marquette High School | A | Bazile | FLENOID | Mai | Westfall | 56.50 | Unverified: |