| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Seckman High School | A | Laird | Ward | Lane | Kundert | 9:50.00 | Unverified: |
| 2 | Lafayette (Wildwood) High School | A | Barnard | Brown | Barnard | Tyson | 9:50.00 | Unverified: |
| 3 | Cor Jesu Academy | A | Morie | McKenzie | Luna | Luna | 10:18.80 | Verified |
| 4 | Webster Groves High School | A | Allen | Nieman | Staab | Davidson | 10:20.00 | Unverified: |
| 5 | Eureka High School | A | Gentilini | Rakey | PEARCE | Crooks | 10:30.00 | Unverified: |
| 6 | Kirkwood High School | A | Durnin | Canatsey | Key | Fleming | 10:33.38 | Unverified: |
| 7 | Lindbergh High School | A | Almamori | Forbes | Smith | Doss | 10:36.00 | Unverified: Jackson Invitational |
| 8 | Parkway Central High School | A | Williams | Qian | Kim | Harris | 11:01.55 | Verified |
| 9 | Ft. Zumwalt North High School | A | Spellazza | Bredenkamp | Johnson | Ebert | 11:03.28 | Verified |
| 10 | Parkway West High School | A | Etchason | Etchason | Paquette | Peters | 11:20.00 | Unverified: |
| 11 | Oakville High School | A | BROWER | Kroener | WETTEROFF | Keck | 11:50.30 | Verified |
| 12 | Hazelwood Central High School | A | Brown | Brown | Shelton | Catching | 12:04.16 | Verified |