| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Northwest (Cedar Hill) High Scho | A | Brandt | Keith | Smith | Poskin | NT | Unverified: |
| 3 | Ft. Zumwalt North High School | A | Jones | Henderson | Futrell | Miller | 4:00.16 | Verified |
| 4 | Maplewood-Richmond Hts. High Sch | A | Hagens | Clark | Carter | Williams | NT | Unverified: |
| 5 | Hazelwood Central High School | A | Atkins | Trotter | Jones | Funches | 1:57.96 | Unverified: Rod Staggs Freshman Sophomore Me |
| 6 | Hazelwood East High School | A | Alexander | Burks | Johnson | Sledge | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | A | Jones | BONNETT | WILLIAMS | ANDERSON | 1:40.40 | Unverified: |
| 2 | Ladue Horton Watkins High School | A | Allen | Patterson | Lucas | Perry | 1:38.80 | Unverified: |
| 3 | Kirkwood High School | A | Smith | Edwards | Webb | Welch | 1:37.00 | Unverified: |
| 4 | Vashon High School | A | Taylor | McField | Green | Aaron | 1.00 | Unverified: |
| 5 | Poplar Bluff High School | A | McFarland | Graham | McBride | Nunn | 1:37.00 | Unverified: |
| 6 | Riverview Gardens High School | A | Thompson | Abel | Anderson | Jones | 1:37.00 | Unverified: Rod Staggs Freshman Sophomore Me |
| 7 | Lafayette (Wildwood) High School | A | TUGGLES | JONES | Preckel | Ems | 1:40.00 | Unverified: |
| 8 | Seckman High School | A | Horton | Maxwell | Rellihan | Harp | 1:43.00 | Unverified: |