| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lindbergh High School | A | Thomas | Kelley | Cintel | Haviland | NT | Unverified: |
| 4 | Oakville High School | A | BROWER | ISLAM | Mueller | WESCOAT | NT | Unverified: |
| 5 | Cor Jesu Academy | A | Stoeckel | Jacoby | Bognar | Graham | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | McCluer North High School | A | Murphy | Hammond | Brown | Goforth | NT | Unverified: |
| 2 | Ft. Zumwalt North High School | A | Hamilton | Kimble | Noel | Jones | 4:58.63 | Verified |
| 3 | Lafayette (Wildwood) High School | A | GOODRICH | Halter | Saddler | Singh | NT | Unverified: |
| 4 | Poplar Bluff High School | A | Williams | Kendle | Dowd | Paumen | 2:03.00 | Unverified: |
| 5 | Northwest (Cedar Hill) High Scho | A | Surratt | Orozco | Shelledy | Stethem | 2:03.00 | Unverified: |
| 6 | Parkway West High School | A | Johnson | Fotheringham | Watts | Grunzinger | 2:13.00 | Unverified: |
| 7 | Vashon High School | A | Taylor | Riggins | Hardieway | Clayton | NT | Unverified: |
| 8 | Parkway South High School | A | Wilson | Bacon | Riegelsberger | Ullom | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood Central High School | A | Butler | Union | Wafford | Simmons | 2:01.24 | Unverified: |
| 2 | Seckman High School | A | Bantle | Formby | Zimmermann | Laird | 1:59.00 | Unverified: |
| 3 | Ladue Horton Watkins High School | A | Buxner | Williams | White | Rogers | 1:56.20 | Unverified: |
| 4 | Riverview Gardens High School | A | Smith | Gladden | Smith | Green | 1.00 | Unverified: |
| 5 | Kirkwood High School | A | Beaumont | Dixon | Wright | Fleming | 1:52.30 | Unverified: |
| 6 | Eureka High School | A | SEITZ | MITCHELL | DEAKIN | SCHNURBUSCH | 1:58.00 | Unverified: |
| 7 | Webster Groves High School | A | Decker | Trevino | Juelich | King | 2:00.00 | Unverified: |
| 8 | Liberty (Wentzville) High School | A | Lynch | Woods | OMara | Omara | 2:02.00 | Unverified: |