| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lindbergh High School | A | Earley | Wheaton | Goss | Watts | NT | Unverified: |
| 2 | Eureka High School | A | PETERSON | Howlett | BADOLATO | Hotop | NT | Unverified: |
| 3 | Marquette High School | A | CODY-JACKSON | ELLIS | BONNETT | Jones | 1:30.20 | Unverified: |
| 4 | Ritenour High School | A | Smith | Watson | Bishop | Robinson | DQ | Unverified: |
| 5 | Christian Brothers College High | A | Hendricks | Love | Tillman | Van | NT | Unverified: |
| 6 | Lafayette (Wildwood) High School | A | Parks | HOEFT | Ems | Marshall | 1:32.50 | Unverified: |
| 7 | St. Louis University High School | A | Chaffee | Bailey | Baur | Moore | NT | Verified |