| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lindbergh High School | A | Brown | Tesch | Duchek | Frantti | NT | Unverified: |
| 4 | Christian Brothers College High | A | McClellan | Stimage | Washington | Jackson | NT | Verified |
| 5 | Chaminade College Preparatory Sc | A | Irving | Love | Licker | D.K.) Weaver | 1:42.40 | Unverified: Chaminade Quad |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Louis University High School | A | Kreuter | Pace | Jordan | Scott | DNS | Unverified: |
| 3 | Ritenour High School | A | Williams | Vaz | Spann | Capone | NT | Unverified: |
| 4 | Lafayette (Wildwood) High School | A | REESE | Retych | VEHIGE | BERRA | 1:35.50 | Unverified: |
| 5 | DeSmet Jesuit High School | A | Terry | Russo | Griffin | Houston | 1:36.55 | Verified |
| 6 | Marquette High School | A | PLEIN | Bergoudian | Clark | LEOPANDO | 1:39.10 | Unverified: |
| 7 | Eureka High School | A | Davis | MCELROY | Robinson | Belloli | 1:39.30 | Unverified: |