| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Christian Brothers College High | A | NT | |||||
| 2 | Eureka High School | A | Belloli | Berry | JOHNSON | PENNINGTON | 3:58.00 | Unverified: |
| 3 | Marquette High School | A | PLEIN | VALENTINE WALLS | Bergoudian | Clark | 3:48.00 | Unverified: |
| 4 | Lafayette (Wildwood) High School | A | REESE | Han | VEHIGE | Retych | NT | Unverified: |
| 5 | DeSmet Jesuit High School | A | Houston | Griffin | Russo | Robinson | 3:45.00 | Unverified: |
| 6 | St. Louis University High School | A | Rammacher | Bognar | Brower-Toland | Fyvie | 3:58.00 | Unverified: |
| 7 | Lindbergh High School | A | Tesch | Adams | Jeanmougin | Freund | NT | Unverified: |