| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SUZUKI, Yoshitaka | SR | Chaminade College Preparatory Sc | 46.00 | Verified |
| 3 | PIONTEK, Samuel | SR | Chaminade College Preparatory Sc | 45.80 | Verified |
| 4 | KING, Carllion | SO | Lafayette (Wildwood) High School | 45.49 | Verified |
| 5 | MOORE, Matthew | SO | St. Louis University High School | 45.74 | Verified |
| 6 | GRIFFIN, STEVION | JR | Marquette High School | 45.90 | Unverified: |
| 7 | ROBINSON, Tyson | JR | Ritenour High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, ROBERT | JR | Marquette High School | 44.16 | Verified |
| 2 | SPAETE JR, Christopher | SR | Christian Brothers College High | 42.54 | Verified |
| 3 | STITH, SANTINO | SO | Lafayette (Wildwood) High School | 42.06 | Verified |
| 4 | BROWN, Allen | JR | Eureka High School | 40.64 | Verified |
| 5 | OLIPHANT, Jacobi | JR | St. Louis University High School | 40.86 | Verified |
| 6 | UMBARGER, RYAN | SR | Eureka High School | 42.27 | Verified |
| 7 | DETERDING, Charles | SR | Lindbergh High School | 43.00 | Verified |
| 8 | HILTON, Wyatt | SO | Lindbergh High School | 44.35 | Verified |