| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JEANMOUGIN, Evan | FR | Lindbergh High School | NT | Unverified: |
| 3 | D.K.) WEAVER, DeKarai | FR | Chaminade College Preparatory Sc | NT | Unverified: |
| 4 | LEZIEANYA, Chinonso | FR | St. Louis University High School | NT | Unverified: |
| 5 | KOBE, Thomas | FR | Lindbergh High School | NT | Unverified: |
| 6 | HAMPTON, Colton | FR | Lindbergh High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, LOGAN | SO | Lafayette (Wildwood) High School | 1:05.00 | Unverified: |
| 2 | PHOENIX, Aiden | SO | St. Louis University High School | 1:03.69 | Verified |
| 3 | WIBBENMEYER, Preston | FR | Eureka High School | 1:02.70 | Unverified: |
| 4 | JOHNSON, WESLEY | SO | Eureka High School | 1:02.38 | Verified |
| 5 | LENGER, George | FR | Eureka High School | 1:02.40 | Unverified: |
| 6 | SCHUTTE, Sauyer | FR | Eureka High School | 1:02.90 | Unverified: |
| 7 | ROGGENBURG, ALEXANDER | SO | Lafayette (Wildwood) High School | 1:04.00 | Unverified: |
| 8 | JOHNSON, Dayton | 8 | Chaminade College Preparatory Sc | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MEISE, Will | JR | Lafayette (Wildwood) High School | 1:01.95 | Verified |
| 2 | BERGOUDIAN, Bruno | SR | Marquette High School | 59.10 | Unverified: |
| 3 | SMALL, Christopher | JR | Marquette High School | 59.00 | Unverified: |
| 4 | BOGNAR, Ethan | SO | St. Louis University High School | 57.54 | Verified |
| 5 | VALENTINE WALLS, ETHAN | SR | Marquette High School | 58.60 | Verified |
| 6 | TUFFLI, Andrew | SO | DeSmet Jesuit High School | 59.00 | Verified |
| 7 | ADAMS, Patrick | SO | Lindbergh High School | 59.86 | Verified |
| 8 | MOLLI, Adam | FR | DeSmet Jesuit High School | 1:02.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONES, JAMELL | SR | Marquette High School | 56.90 | Unverified: |
| 2 | JORDAN, D'Corey | SO | St. Louis University High School | 56.15 | Verified |
| 3 | JACKSON, Roman | FR | Christian Brothers College High | 55.20 | Unverified: |
| 4 | LEICHT, Jude | 5 | Christian Brothers College High | 54.94 | Verified |
| 5 | RETYCH, Rylan | JR | Lafayette (Wildwood) High School | 55.00 | Unverified: |
| 6 | TERRY, Justin | SO | DeSmet Jesuit High School | 55.74 | Unverified: |
| 7 | JONES, Darian | FR | Christian Brothers College High | 56.22 | Unverified: |
| 8 | SOMMERVILLE, Tyler | FR | Christian Brothers College High | 57.18 | Unverified: |