| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lindbergh High School | A | Estrada | Goss | Birk | Watts | NT | Unverified: |
| 2 | St. Louis University High School | A | Chaffee | Bailey | Baur | Humphrey | NT | Verified |
| 3 | Eureka High School | A | Brown | ANDERSON | BADOLATO | Howlett | NT | Unverified: |
| 4 | Christian Brothers College High | A | Van | Hendricks | McClellan | Love | NT | Unverified: |
| 5 | Lafayette (Wildwood) High School | A | Parks | King | STITH | LEWIS | 44.00 | Unverified: |
| 6 | Marquette High School | A | BONNETT | Jones | CODY-JACKSON | ELLIS | 44.60 | Unverified: |
| 7 | Chaminade College Preparatory Sc | A | Fields | Taylor | Gray | Collins | NT | Unverified: Chaminade Quad |