| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | A | NOLDON | PLEIN | Bergoudian | Clark | 48.50 | Unverified: |
| 2 | Christian Brothers College High | A | Williams | Morris | Cothrine | Washington | 47.94 | Verified |
| 3 | Eureka High School | A | Hasler | Robinson | Belloli | KOHL | DNS | Unverified: |
| 4 | DeSmet Jesuit High School | A | Byerley | King | McCaster | Williams | 46.02 | Verified |
| 5 | Lafayette (Wildwood) High School | A | REESE | TINSLEY | Preckel | Tuggles | 46.50 | Unverified: |
| 6 | St. Louis University High School | A | Kreuter | Pace | Phoenix | Bennett | DNS | Unverified: |
| 7 | Chaminade College Preparatory Sc | A | Brown | Licker | Love | Irving | 48.50 | Unverified: Chaminade Quad |
| 8 | Lindbergh High School | A | Sengupta | Freels | Engbert | Lin | DNS | Unverified: |