| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | McCluer North High School | A | Collins | Davis | Myers | Thompson | NT | Unverified: |
| 4 | Hazelwood Central High School | A | Bright | Anthony | Martin | Thornton | NT | Unverified: |
| 5 | KIPP St. Louis | A | Pate | Kemp | Potter | Burch | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vashon High School | A | Cain III | Brown | Taylor | McField | NT | Unverified: |
| 3 | Riverview Gardens High School | A | Graham | McClain | McGhee | Shelton | NT | Unverified: |
| 4 | Confluence Prep Academy Charter | A | Moore | Dailey-Jones | Cameron | Lance | NT | Unverified: |
| 5 | Gateway High School | A | walker | Washington | Shelton | Bland | NT | Unverified: |
| 6 | STEAM Academy at McCluer South-B | A | Smith | Johnson | Grimes | Hines | NT | Unverified: |
| 7 | Hazelwood East High School | A | III | Johnson | Shakoor | Sledge | NT | Unverified: |