| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | McCluer North High School | A | Buckley | Murphy | Goforth | Hart | NT | Unverified: |
| 4 | Hazelwood West High School | A | Cuthlow-Davis | Jones | Young | Squires | NT | Unverified: |
| 5 | Confluence Prep Academy Charter | A | Guy | Daniels | Huntley | Waters | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vashon High School | A | Simpson-Bey | Mosely | Evans | Boyd | NT | Unverified: |
| 2 | Hazelwood Central High School | A | Mckenzie | Reed | Wilkins | Madison | NT | Unverified: |
| 3 | STEAM Academy at McCluer South-B | A | 54.17 | Unverified: | ||||
| 4 | East St. Louis (Sr.) | A | Glenn | Graham | Jones | Lampley | 48.80 | Unverified: |
| 5 | Hazelwood East High School | A | Carter | Easley | Fitch | Spates | 51.15 | Unverified: |
| 7 | Riverview Gardens High School | A | Harris-Grayer | Perkins | Lewis | Coleman | NT | Unverified: |