| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | COFIELD, De'Oni | K C Elite Competition | NT | ||
| 4 | KRAFT, Addison | Unattached | NT | ||
| 5 | TAYLOR, Gia | Palestine Prowlers Sports Asso | NT | ||
| 6 | ODEMS, Saidee | K C Elite Competition | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RONE, Neveah | KC Dark Knights | 36.03 | ||
| 2 | BARKER, Danay | ST Louis Scorpions Track Club | 33.85 | ||
| 3 | RIMPSON, Layla | Palestine Prowlers Sports Asso | 32.40 | ||
| 4 | WHITEHEAD, Justice | Blues Track Club of Greater St | 31.60 | ||
| 5 | BURROW, Paityn | Kersh Strong Elite Sports Acad | 32.31 | ||
| 6 | FERGIE, Alice | Hounds | 32.80 | ||
| 7 | HOWELL, Ianna | Whatever It Takes | 34.75 | ||
| 8 | GRIFFIN, Rozlynn | KC Dark Knights | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLEMONS, Aniya | Alton Blaze | 30.00 | ||
| 2 | AHMED, Aniyah | Blues Track Club of Greater St | 29.89 | ||
| 3 | THOMAS, Brooklyn | Active Strength Track Club | 29.70 | ||
| 4 | BOYD, Alexis | ST Louis Scorpions Track Club | 29.10 | ||
| 5 | BASS, Gabrielle | Northland Track Club | 29.50 | ||
| 6 | SIMS, Justice | ST Louis Scorpions Track Club | 29.79 | ||
| 7 | BECKNER, Emily | Cornerstone Athletics | 30.00 | ||
| 8 | SHANKS, Dionne | Blues Track Club of Greater St | 30.50 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONES, Samantha | Northland Track Club | 28.80 | ||
| 2 | HOUPE, Jenae | kcrebels | 28.64 | ||
| 3 | CLARK, Saige | KC Swag | 28.50 | ||
| 4 | CHATMAN, Tamia | Blues Track Club of Greater St | 27.20 | ||
| 5 | WOODS, London | Active Strength Track Club | 28.00 | ||
| 6 | TUCKER, Celiciana | ST Louis Scorpions Track Club | 28.57 | ||
| 7 | HUNZIKER, Kiptyn | Hot Feet Track Club LLC | 28.64 | ||
| 8 | CALDWELL, Jyairra | Della Lamb Track Club | 28.89 |