| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | KALIMULLAH, Nadiya | KC Swag | NT | ||
| 3 | GALLO, Ellie | Tallawah Track and Field | NT | ||
| 4 | FAIR, Jaylah | KC Dark Knights | NT | ||
| 5 | JONES, Alana | Columbia Supreme Track Club | NT | ||
| 6 | TONEY, Rhiyan | Active Strength Track Club | NT | ||
| 7 | CARLYLE, Melrose | Unattached | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Royalty | KC Dark Knights | NT | ||
| 2 | ARCALA, Clara | Blue Thunder Track Club | 41.00 | ||
| 3 | MURRAY, Uniqui | Active Strength Track Club | 35.00 | ||
| 4 | HEARD, Layla | Ultimate Speed Academy | 34.71 | ||
| 5 | NEWBILL, Aria | Team G | 35.00 | ||
| 6 | FREEMAN, Zoey | Ultimate Speed Academy | 37.89 | ||
| 7 | WALKER, Symphanee | K C Elite Competition | NT | ||
| 8 | ORIE, Ashton | KC Swag | NT |