| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMPSON, Vashawn | Columbia Supreme Track Club | NT | ||
| 2 | PARKER, Jackson | Unattached | 1:10.00 | ||
| 3 | ATKINS, Jaylen | JWBolt | 29.23 | ||
| 4 | SAIN, Durand | Greyhounds Track Club | 24.60 | ||
| 5 | BALLINGER, Garrett | Unattached | 27.00 | ||
| 6 | POTTS, Josiah | Royal Knights | 30.00 | ||
| 7 | HUNTER, Damarion | Columbia Supreme Track Club | NT | ||
| 8 | TILLER, Kai | JWBolt | NT |