| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Finn | Team G | NT | ||
| 2 | STONE, Dezmond | Blues Track Club of Greater St | NT | ||
| 3 | FUEMMELER, Jordan | Team G | NT | ||
| 4 | LADAGE, Zak | Unattached | 1:00.00 | ||
| 5 | ARMSTRONG, Jonathan | Dashers Track and Sports | 1:05.00 | ||
| 6 | MUSHATT, Elnailo | kcrebels | NT | ||
| 7 | LARUE, Lucas | Kersh Strong Elite Sports Acad | NT | ||
| 8 | COLE, Kendrick | Excelerate Phenoms | NT |