| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | DENOON, Gwendolyn | Tallawah Track and Field | NT | ||
| 4 | JONES, Talia | Royal Knights | NT | ||
| 5 | HIERS, Hannah | Tallawah Track and Field | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HENDRICKS, Kristen | Unattached | NT | ||
| 2 | QIN, Charlotte | Tempo Track Club | 1:00.00 | ||
| 3 | MORETINA, Mackenzie | Pure Talent Elite Track and Fi | 59.00 | ||
| 4 | BROWN, Avery | Tallawah Track and Field | 57.00 | ||
| 5 | GOODWIN, Nevaeh | Palestine Prowlers Sports Asso | 58.00 | ||
| 6 | BOEHM, Brooke | Pony Express Athletic Performa | 59.50 | ||
| 7 | BREDENKAMP, Alivia | Kersh Strong Elite Sports Acad | 1:10.00 |