| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 5 | JOHNSON, Malaya | Boston Mountain Athletics | 2:16.21 | ||
| 6 | BRYANT, Nevaeh | Alton Blaze | 2:25.00 | ||
| 7 | VERSLUES, Lauren | Unattached | 2:26.30 | ||
| 8 | SNEE, Brooke | VAST Track & Field and Cross C | 2:29.00 | ||
| 9 | MEYERKORTH, Ella | Pony Express Athletic Performa | 2:33.00 | ||
| 10 | COLEMAN, Lauryn | Excelerate Phenoms | 2:34.03 | ||
| 11 | HARRIS, Armani | Unattached | 2:38.00 | ||
| 12 | FAIR, Mahogany | Cahokia Track Club | 3:50.00 |