| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WRIGHT, Amerie | KC Swag | 2:30.00 | ||
| 2 | WILLIAMS, Lauryn | Excelerate Phenoms | 2:30.48 | ||
| 3 | KUDZIA, Kelsie | Hot Feet Track Club LLC | 2:32.59 | ||
| 4 | PATRICIO, Kailen | Excelerate Phenoms | 2:37.73 | ||
| 5 | OMAR, Luul | Columbia Supreme Track Club | 2:44.76 |