| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RENO, Tommie | St Louis PAL | 2:29.07 | ||
| 2 | BARNETT, Aiden | Excelerate Phenoms | 2:29.30 | ||
| 3 | HUNTER, Dre'Shawn | Columbia Supreme Track Club | 2:29.68 | ||
| 4 | PONTILLO, Austin | Hot Feet Track Club LLC | 2:30.00 | ||
| 5 | CLARK, Shamaur | Blues Track Club of Greater St | 2:32.18 | ||
| 6 | GONZALEZ, Ian | Hot Feet Track Club LLC | 2:35.00 | ||
| 7 | MASON, Luke | Blue Thunder Track Club | 2:35.00 | ||
| 8 | CUNNINGHAM, Legend | ST Louis Scorpions Track Club | 2:38.25 | ||
| 9 | JENKINS, Carter | Ultimate Speed Academy | 2:47.57 | ||
| 10 | REEVES, Nolan | Active Strength Track Club | 2:54.32 | ||
| 11 | CRUZ, Charles | Blue Thunder Track Club | 3:02.10 | ||
| 12 | CHATMAN, Douglas | Ultimate Speed Academy | NT | ||
| 13 | BIERRIA, Joseph | Palestine Prowlers Sports Asso | NT |