| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUNZIKER, Kiptyn | Hot Feet Track Club LLC | 2:30.00 | ||
| 2 | MOIBI, Arianna | Hot Feet Track Club LLC | 2:37.00 | ||
| 3 | COOK, Catherine | Hot Feet Track Club LLC | 2:39.10 | ||
| 4 | LONG, Bella | Blue Thunder Track Club | 2:41.00 | ||
| 5 | KALIMULLAH, Emani | KC Swag | 2:45.00 | ||
| 6 | JEFFERSON, Kamryn | ST Louis Scorpions Track Club | 2:51.36 | ||
| 7 | HOOVER, Maya | Blue Thunder Track Club | 3:04.26 | ||
| 8 | ARCALA, Elaina | Blue Thunder Track Club | 3:16.00 | ||
| 9 | ZENTZ, Annabelle | Blue Thunder Track Club | 3:21.00 |