| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KINWORTHY, Millie | Blue Thunder Track Club | 3:17.98 | ||
| 2 | ARONHALT, Avalynn | Blue Thunder Track Club | 3:29.09 | ||
| 3 | FOSTER, Rosalie | Blue Thunder Track Club | 3:55.00 | ||
| 4 | LOYD, Lauren | Hounds | 4:08.13 | ||
| 5 | ZENTZ, Amelia | Blue Thunder Track Club | 4:09.70 | ||
| 6 | BOWEN, Lydia | Hounds | 4:37.56 | ||
| 7 | JENKINS, Ivy | Ultimate Speed Academy | NT | ||
| 8 | HAAR, Priya | Tallawah Track and Field | NT | ||
| 9 | HAAR, Autumn | Tallawah Track and Field | NT |