| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Chaminade College Preparatory Sc | A | Chris) Funches | Collins | D.K.) Weaver | Tim) Bailey | 1:41.28 | Verified |
| 4 | Whitfield High School | A | Taylor | Schulte | Wortham III | Carter | 1:38.56 | Verified |
| 5 | Lutheran South High School | A | Grus | Hackett | Hoehner | Woods | 1:39.68 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Affton High School | A | Mitchell | Williams | Lovelace | Moehlenbrock | 1:37.56 | Verified |
| 3 | Principia High School | A | Ejimadu | Dannull | Mugabe | Warrick | 1:36.86 | Verified |
| 4 | Gateway High School | A | Calvin | Clay | Jones | Lawrence | 1:36.45 | Verified |
| 5 | Brentwood High School | A | Hawkins | Lane | Thompson | Phillips | 1:36.68 | Verified |
| 6 | Clayton High School | A | Aningo | Anderson-Jennings | Chatman | Handzic-Smith | 1:37.03 | Verified |
| 7 | Vianney High School | A | Pullam | Ivory | Culleton | Selsor | 1:38.03 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | John Burroughs High School | A | Dharmarajan | Goffstein | Campbell | Foote | 1:35.78 | Verified |
| 2 | St. Mary's (St. Louis) High Scho | A | Strong | Washington | Hendricks | Roberts | 1:33.02 | Verified |
| 3 | STEAM Academy at McCluer South-B | A | Coleman | Moreland-Bell | Smith | Grimes | 1:29.05 | Unverified: STEAM ACADEMY Spring Invitationa |
| 4 | MICDS | A | Clay | Muhammad | Weaver | Harris | 1:27.32 | Verified |
| 5 | Lutheran North High School | A | Bass | Van Hook | Carter | Clark | 1:28.89 | Verified |
| 6 | Hazelwood Central High School | A | Martin | Atkins | Williams | Williams | 1:31.97 | Verified |
| 7 | Bayless High School | A | Miller | Patton | Jones | Uebari | 1:35.03 | Verified |
| 8 | Lift for Life Academy Charter Hi | A | Hightower | Jones | Ridgel | Young | DQ | Verified |