| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Gateway Science Charter | A | Axe | Spieckerman | Villarreal | Jeltes | 4:12.52 | Verified |
| 4 | Gateway High School | A | Shelton | Warren | Bland | Spencer | 4:07.33 | Verified |
| 5 | Vianney High School | A | Turmelle | Miesner | Boze | Lindsey | 4:10.58 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bayless High School | A | Ibrahim | Miller | Saville | Hulsey | 3:58.90 | Verified |
| 2 | University City High School | A | Klein | Sims | Fleming | Holman | 3:53.00 | Unverified: |
| 3 | Lift for Life Academy Charter Hi | A | Jones | Hightower | Rogers | Landers | 3:46.27 | Verified |
| 4 | Chaminade College Preparatory Sc | A | D.K.) Weaver | Piontek | Suzuki | Tim) Bailey | 3:40.17 | Verified |
| 5 | Principia High School | A | Kawesa | Warrick | Ostler | Udoekong | 3:41.91 | Verified |
| 6 | Affton High School | A | Lovelace | Pashkov | Mitchell | Williams | 3:46.93 | Verified |
| 7 | Priory High School | A | Britt | Mueller | Hamill | Schmerold | 3:58.12 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Whitfield High School | A | Butler | Brungart | Courtney | Flood | 3:35.63 | Verified |
| 2 | St. Mary's (St. Louis) High Scho | A | Hendricks | Washington | Ford | Rwabusisi | 3:35.17 | Unverified: |
| 3 | MICDS | A | Clay | McCarthy | Muhammad | Weaver | 3:30.14 | Verified |
| 4 | Lutheran North High School | A | Carter | Clark | Johnson | Harvey | 3:29.59 | Verified |
| 5 | STEAM Academy at McCluer South-B | A | Moreland-Bell | Gregory | Evans | Parker | NT | Unverified: |
| 6 | Lutheran South High School | A | Samking | Buscher | Hickerson | Snoke | 3:35.12 | Verified |
| 7 | Hazelwood Central High School | A | Johnson | Logan | Hunter | Anthony | 3:35.44 | Verified |
| 8 | John Burroughs High School | A | Tao | Knispel | Riley | Doty | 3:36.77 | Verified |