| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BARNES, Marrione | FR | Lutheran North High School | 2:19.19 | Verified |
| 2 | IBRAHIM, Sabiri | JR | Bayless High School | 2:24.35 | Verified |
| 3 | ROCHE, Andrew | JR | Vianney High School | 2:24.70 | Verified |
| 4 | JONES-SPANN, Azaar | FR | St. Mary's (St. Louis) High Scho | 2:25.42 | Verified |
| 5 | CHOU, Isaac | SO | Vianney High School | 2:26.19 | Verified |
| 6 | EDWARDS, Caden | SO | Whitfield High School | 2:28.55 | Verified |
| 7 | AXE, Hugo | SR | Gateway Science Charter | 2:29.81 | Verified |
| 8 | BASS, Andrew | SR | Gateway High School | 2:31.74 | Verified |
| 9 | CHARMLEY, Elijah | SO | Whitfield High School | 2:35.77 | Verified |
| 10 | HALL, London | FR | Lift for Life Academy Charter Hi | 2:36.40 | Verified |
| 11 | BOSTIC-VALERIO, Luca | SO | Brentwood High School | 2:39.15 | Verified |
| 12 | REEDS, William | SO | Brentwood High School | 2:43.25 | Verified |
| 13 | MCDONALD, Kamal | JR | Lift for Life Academy Charter Hi | 2:47.75 | Verified |
| 14 | ALNUSOUR, Ismael | FR | Gateway Science Charter | 2:48.82 | Verified |
| 15 | OMEROVIC, Emrah | SR | Affton High School | 2:49.65 | Verified |
| 16 | ALLEN, David | FR | Principia High School | NT | Unverified: |
| 17 | EWALD, Andrew | SO | Lutheran South High School | NT | Unverified: |
| 18 | WATT HOVEN, Zane Oliver | JR | University City High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SNOKE, Adam | SR | Lutheran South High School | 1:55.61 | Unverified: Corey Siebert -Lakeshia Finch T& |
| 2 | HAMILL, Patrick | JR | Priory High School | 2:01.13 | Verified |
| 3 | ELLIS, Aiden | JR | Hazelwood Central High School | 2:02.10 | Verified |
| 4 | MCDANIEL, Josiah | SR | Hazelwood Central High School | 2:06.34 | Verified |
| 5 | TAO, Chris | FR | John Burroughs High School | 2:06.44 | Unverified: |
| 6 | KLEIN, Elias | SR | University City High School | 2:06.55 | Verified |
| 7 | JOHNSON, Sylvester | JR | Lutheran North High School | 2:07.00 | Unverified: Metro League Championships - Var |
| 8 | KNISPEL, Samuel | SR | John Burroughs High School | 2:08.27 | Unverified: |
| 9 | MUELLER, Matthew | JR | Priory High School | 2:11.02 | Unverified: |
| 10 | WOLAK, Ryan | SR | Chaminade College Preparatory Sc | 2:11.28 | Verified |
| 11 | WILLIAMS, Deryck | SR | MICDS | 2:11.71 | Verified |
| 12 | FIELDS, Leonard | JR | STEAM Academy at McCluer South-B | 2:12.27 | Verified |
| 13 | THOMAS, Holland | JR | Principia High School | 2:14.67 | Verified |
| 14 | HOETTE, Charles | SR | Clayton High School | 2:15.14 | Verified |
| 15 | LOVELACE, Josiah | JR | Affton High School | 2:16.42 | Verified |
| 16 | WASHINGTON, Juan | SO | Gateway High School | 2:17.62 | Verified |
| 17 | BAKER, Luke | JR | Clayton High School | 2:17.64 | Verified |
| 18 | FENDLER, Will | FR | MICDS | 2:18.53 | Verified |